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protein kinase 3  (Proteintech)


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    Structured Review

    Proteintech protein kinase 3
    Protein Kinase 3, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 219 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+3/PRKX+Antibody/pmc13672626-96-27-48
    Average 96 stars, based on 219 article reviews
    protein kinase 3 - by Bioz Stars, 2026-11
    96/100 stars

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    other:

    Article Title: Ershen Dan attenuates atherosclerosis by modulating the NOTCH1/NF-κB/NLRP3 signaling pathway to suppress M1 macrophage polarization
    Article Snippet: Neurogenic locus notch homolog protein 1 (NOTCH1; 10062-2-AP; Proteintech, China), hairy and enhancer of split-1 (HES1; A0925; ABclonal, China), NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3; 30109-1-AP; Proteintech, China), nuclear factor (NF)-κB p65 (10745-1-AP; Proteintech, China), phospho-NF-κB p65 (82335-1-RR; Proteintech, China), and NADPH oxidase 2 (NOX2; 19013-AP; Proteintech, China) were used.

    Article Title: Erianin induces GSDMD-dependent pyroptosis and synergistically enhances doxorubicin efficacy via the PI3K/AKT signaling pathway in diffuse large B-cell lymphoma
    Article Snippet: The following antibodies were purchased from Proteintech: NOD-like receptor thermal protein domain associated protein 3 (NLRP3, 1:1000), β-actin (1:5000), S100A9 (1:1000), and Ki67 (1:1000).

    Article Title: Tetrastigma hemsleyanum Diels et Gilg Flavonoids Against Acute Lung Injury Via Block NLRP3 Inflammation
    Article Snippet: The antibodies of IL‐18 (10663‐1‐AP) and NOD‐like receptor thermal protein domain‐associated protein 3 (NLRP3, 68102‐1‐lg) were bought from Proteintech (Wuhan, China).

    Immunocytochemistry:

    Article Title: The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons
    Article Snippet: Cells were then permeabilized and blocked with 3% bovine serum albumin (Sangon Biotech, Shanghai, China) or 5% donkey serum in phosphate buffered saline/Gly with 0.3% Triton X-100 for approximately 1 hour, followed by incubation with primary antibodies for 3 hours and then with secondary antibodies for 1 hour at room temperature. .. The following primary antibodies were used for immunocytochemistry: postsynaptic density-95 (PSD-95, mouse, 1:400, NeuroMab, Davis, CA, USA, Cat# K28/74, RRID: AB_2877478), kinesin family member 21A (KIF21A, rabbit, 1:100, Sigma-Aldrich, Cat# PA5-63572, RRID: AB_2643096), human influenza hemagglutinin (HA, mouse, 1:500, Abcam, Cambridge, UK, Cat# ab130275, RRID: AB_11156884), end-binding protein 3 (EB3, rabbit, 1:200, Proteintech, Cat# 23974-1-AP, RRID: AB_2879385), Piccolo (chicken, 1:500, SySy, Göttingen, Germany, Cat# 142116, RRID: AB_2661778), GluA1 (rabbit, 1:500, Alomone Labs, Jerusalem, Israel, Cat# AGC-004, RRID:AB_2039878), Homer1 (rabbit, 1:300, Proteintech, Cat# 12433-1-AP, RRID: AB_2295573), and KN motif and ankyrin repeat domain-containing protein 1 (KANK1, rabbit, 1:800, Thermo Fisher Scientific, Waltham, MA, USA, Cat# PA5-34533, RRID: AB_2551885). .. The secondary antibodies used were as follows: Alexa 647 donkey anti-rabbit (Jackson ImmunoResearch, Lancaster, PA, USA, Cat# 711-605-152, RRID: AB_2492288), Alexa 647 donkey anti-mouse (Jackson ImmunoResearch, Cat# 715-605-151, RRID: AB_2340863), Alexa cyanine3 (cy3) donkey anti-rabbit (Jackson ImmunoResearch, Cat# 711-165-152, RRID: AB_2307443), Alexa cy3 donkey anti-mouse (Jackson ImmunoResearch, Cat# 715-165-151, RRID: AB_2315777), unconjugated donkey anti-mouse antibody (ackson ImmunoResearch, Cat# 715-005-151, RRID: AB_2340759), and donkey anti-rabbit (ackson ImmunoResearch, Cat# 711-005-152, RRID: AB_2340585) labeled with Cy TM 3B NHS Ester, GE Healthcare, Chicago, IL, USA).

    Hemagglutination Assay:

    Article Title: The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons
    Article Snippet: Cells were then permeabilized and blocked with 3% bovine serum albumin (Sangon Biotech, Shanghai, China) or 5% donkey serum in phosphate buffered saline/Gly with 0.3% Triton X-100 for approximately 1 hour, followed by incubation with primary antibodies for 3 hours and then with secondary antibodies for 1 hour at room temperature. .. The following primary antibodies were used for immunocytochemistry: postsynaptic density-95 (PSD-95, mouse, 1:400, NeuroMab, Davis, CA, USA, Cat# K28/74, RRID: AB_2877478), kinesin family member 21A (KIF21A, rabbit, 1:100, Sigma-Aldrich, Cat# PA5-63572, RRID: AB_2643096), human influenza hemagglutinin (HA, mouse, 1:500, Abcam, Cambridge, UK, Cat# ab130275, RRID: AB_11156884), end-binding protein 3 (EB3, rabbit, 1:200, Proteintech, Cat# 23974-1-AP, RRID: AB_2879385), Piccolo (chicken, 1:500, SySy, Göttingen, Germany, Cat# 142116, RRID: AB_2661778), GluA1 (rabbit, 1:500, Alomone Labs, Jerusalem, Israel, Cat# AGC-004, RRID:AB_2039878), Homer1 (rabbit, 1:300, Proteintech, Cat# 12433-1-AP, RRID: AB_2295573), and KN motif and ankyrin repeat domain-containing protein 1 (KANK1, rabbit, 1:800, Thermo Fisher Scientific, Waltham, MA, USA, Cat# PA5-34533, RRID: AB_2551885). .. The secondary antibodies used were as follows: Alexa 647 donkey anti-rabbit (Jackson ImmunoResearch, Lancaster, PA, USA, Cat# 711-605-152, RRID: AB_2492288), Alexa 647 donkey anti-mouse (Jackson ImmunoResearch, Cat# 715-605-151, RRID: AB_2340863), Alexa cyanine3 (cy3) donkey anti-rabbit (Jackson ImmunoResearch, Cat# 711-165-152, RRID: AB_2307443), Alexa cy3 donkey anti-mouse (Jackson ImmunoResearch, Cat# 715-165-151, RRID: AB_2315777), unconjugated donkey anti-mouse antibody (ackson ImmunoResearch, Cat# 715-005-151, RRID: AB_2340759), and donkey anti-rabbit (ackson ImmunoResearch, Cat# 711-005-152, RRID: AB_2340585) labeled with Cy TM 3B NHS Ester, GE Healthcare, Chicago, IL, USA).

    Western Blot:

    Article Title: Caffeic acid ameliorates intestinal barrier injury to attenuate acute pancreatitis via inhibiting GSDMD-mediated pyroptotic pathway.
    Article Snippet: Ethnopharmacological relevance: Acute pancreatitis (AP) is a common and critical disease in clinical practice.. Salvia miltiorrhiza Bunge (Danshen), a traditional Chinese medicine, has been used in the clinical treatment of AP due to its ability to regulate inflammatory factors, prevent oxidation, and improve microcirculation, achieving certain therapeutic effects.. In recent years, most research on the active ingredients of Salvia miltiorrhiza Bunge for the treatment of AP has focused on fat-soluble components such as tanshinone, while water-soluble components, such as phenolic acids have been studied much less.

    Binding Assay:

    Article Title: SERCA2 dysfunction stimulates inflammation and causes pulmonary vascular remodeling by downregulating PPARγ/PGC1α/Nrf2.
    Article Snippet: Our prior research has demonstrated that dysfunction of sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 2 (SERCA2) is a common causal factor in the development of pulmonary vascular remodeling, as it accelerates cell proliferation and migration in pulmonary artery smooth muscle cells (PASMCs).. Inflammation is known to play a critical role in pulmonary vascular remodeling.. However, the contribution of SERCA2 dysfunction to inflammation within the pulmonary vasculature has not been previously reported.

    Article Title: Artemether ameliorates type 1 diabetes mellitus by modulating glycolipid metabolism in skeletal muscle
    Article Snippet: .. Antibodies for citrate synthase, isocitrate dehydrogenase 3A, oxoglutarate dehydrogenase, hexokinase 2, pyruvate kinase muscle isoform, phosphofructokinase muscle isoform, pyruvate dehydrogenase kinase 4, acyl-coenzyme A (acyl-CoA) dehydrogenases (ACADVL, ACADL, ACADM, ACADS), glyceraldehyde-3-phosphate dehydrogenase, and fatty acid binding protein 3 were sourced from Proteintech. .. Pyruvate dehydrogenase, phosphorylated pyruvate dehydrogenase (Ser293), and pyruvate dehydrogenase phosphatase 1 antibodies were sourced from Cell Signaling Technology.

    Incubation:

    Article Title: PINK1 overexpression suppresses p38 MAPK/NF-κB signaling to attenuate chondrocyte senescence in osteoarthritis
    Article Snippet: Cells were lysed using RIPA buffer (Beyotime Institute of Biotechnology) supplemented with a protease inhibitor cocktail on ice for 30 min. .. The membranes were blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4°C with the following primary antibodies: GAPDH (1:50,000; cat. no. 66004-1-Ig), β-tubulin (1:3,000; cat. no. 10094-1-AP), p65 (cat. no. 10745-1-AP), phosphorylated (p-)p65 (cat. no. 82335-1-RR), p38 (cat. no. 14064-1-AP), p-p38 (all Proteintech Group, Inc.; cat. no. 28796-1-AP), MMP-3 (Affinity Biosciences; cat. no. AF0217), iNOS (inducible nitric oxide synthase) (cat. no. 18985-1-AP), P16 (both Proteintech Group, Inc.; cat. no. 10883-1-AP), P21 (Affinity Biosciences; cat. no. AF6290), PINK1 (cat. no. 23274-1-AP), TUFm (cat. no. 26730.1.AP), BNIP3L (Bcl-2) interacting protein 3-like) (cat. no. 12986-1-AP), p62 (cat. no. 18420-1-AP) and LC3B (Microtubule-associated protein 1 light chain 3B) (all 1:1,000; all Proteintech Group, Inc.; cat. no. 18725.1-AP). ..



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    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, <t>LC3</t> II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
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    96
    Proteintech protein kinase 3
    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, <t>LC3</t> II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
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    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, <t>LC3</t> II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.
    Bradford Protein Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Journal: Journal of Sport and Health Science

    Article Title: Weightlifting outperforms voluntary wheel running for improving adiposity and insulin sensitivity in obese mice

    doi: 10.1016/j.jshs.2025.101100

    Figure Lengend Snippet: Resistance exercise exceeds the benefits of endurance exercise in ameliorating metabolic dysfunction. Following 8 weeks of diet and exercise interventions, all mice were assessed for their metabolic function by GTT, ITT, and skeletal muscle response of Akt and AS160 phosphorylation to injection of insulin measured by Western blot. (A–C) HOMA-IR taken after an overnight fast for baseline glucose and insulin. (D and E) GTT from 0–120 min and calculated AUC; colored * indicates significant difference from NC-SED. (F and G) ITT from 0–60 min and calculated AUC; colored * indicates significant difference from NC-SED. (H–N) pAkt stimulation, AS160 S318, and AS160 T642 in hindlimb muscles before and after insulin injection and the pre–post ∆ in phosphorylation. (O and P) Total and phosphorylated 4E-BP1. (Q–T) Western results for Raptor, COX4, LC3 II/I, and ubiquitin staining. Representative western blot images inset right. Data presented as mean ± standard error of the mean. Statistical analysis performed by analysis of variance between groups: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001. NC-SED n : 8–16 (white); HFD-SED n : 8–18 (red); HFD-R EX n : 8–16 (blue); HFD-E EX n : 8–15 (green). 4E-BP1 = Eukaryotic translation initiation factor 4E binding protein; Akt = protein kinase B; AS160 = Akt substrate 160; COX4 = cytochrome c oxidase 4; CS = citrate sythase; E EX = endurance exercise; GAPDH = glyceraldehyde 3 phosphate dehydrogenase; GTT = glucose tolerance test; HFD = high fat diet; HOMA-IR = homeostatic model assessment for insulin resistance; iAUC = integrated area under the curve; ITT = insulin tolerance test; LC3 II/I = microtubule-associated protein light chain 3; NC = normal chow; pAkt = phospho-Akt; R EX = resistance exercise; SED = sedentary; Ub = ubiquitin.

    Article Snippet: Primary antibodies used for analysis were from Cell Signaling Technologies (Danvers, MA, USA) and diluted 1:1000 unless otherwise stated as follows: protein kinase B (Akt; 1:500; #4691; Cell Signaling Technologies), phospho-Akt (pAkt) S473 (1:500; #9271; Cell Signaling Technologies), Ubiquitin (#3933; Cell Signaling Technologies), microtubule-associated protein light chain 3 (LC3 II/I; #4018; Cell Signaling Technologies), cytochrome c oxidase subunit 4(COX4; #11967; Cell Signaling Technologies), Akt substrate 160 (AS160 S318; #8619; Cell Signaling Technologies), AS160 T642 (#8881; Cell Signaling Technologies), eukaryotic translation initiation factor 4E binding protein (4E-BP1; #9452; Cell Signaling Technologies), and glyceraldehyde 3 phosphate dehydrogenase (GAPDH; #2118; Cell Signaling Technologies).

    Techniques: Phospho-proteomics, Injection, Western Blot, Muscles, Ubiquitin Proteomics, Staining, Binding Assay